![]() ![]() For E2F1 and histone ChIP-seq, the chromatin was immunoprecipitated with appropriate amounts of antibody. Extracted molecule genomic DNA Extraction protocol Cells were cross-linked then sonicated to generate chromatin fragment sizes between 250-500bp. UC-MSCs were cultured in Dulbecco’s modified Eagle’s medium containing high glucose which was further supplemented with 20% fetal bovine serum, 1 X Insulin-Transferrin-Selenium G, 5 ng/ml basic fibroblast growth factor, 1 X Antibiotic/antimycotic mix and 0.1 mmol/l beta-mercaptoethanol. Scope: Format: Amount: GEO accession: Status Public on Title ID44.H3K4me1 Sample type SRA Source name umbilical cord wharton's jelly Organism Characteristics chip antibody: H3K4me1 (2ug Abcam, AB8895) number of cells: 1.5 million cell type: Mesenchymal stem cells agasga: AGA Growth protocol UC-MSCs were cultured in Dulbecco’s modified Eagle’s medium containing high glucose which was further supplemented with 20% fetal bovine serum, 1 X Insulin-Transferrin-Selenium G, 5 ng/ml basic fibroblast growth factor, 1 X Antibiotic/antimycotic mix and 0.1 mmol/l beta-mercaptoethanol. GEO help: Mouse over screen elements for information.
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